Journal: Endocrinology and Metabolism
Article Title: Development of a Long-Acting Follicle-Stimulating Hormone Using Serum Albumin Fab-Associated Technology for Female Infertility
doi: 10.3803/EnM.2024.2090
Figure Lengend Snippet: Effects of anti-serum albumin Fab-associated (SAFA)-follicle-stimulating hormone (FSH) in cell line experiments. (A) Cyclic adenosine monophosphate (cAMP) production, (B) estradiol synthesis, (C) human cytochrome P450 family 19 subfamily A member 1 (hCYP19α1) expression, and (D) human steroidogenic acute regulatory protein (hSTAR) expression in human ovarian granulosa (KGN) cells treated with indicated doses of recombinant human follicle-stimulating hormone (rhFSH) or SAFA-FSH. Data are representative of three independent experiments and are presented as mean±standard error of the mean.
Article Snippet: A human ovarian granulosa (KGN) cell line (RCB1154, RIKEN BioResource Research Center, Kyoto, Japan), were maintained in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA) and 1% penicillin/streptomycin (Hyclone) in a humidified atmosphere containing 5% CO 2 and 95% air at 37°C.
Techniques: Expressing, Recombinant